Greiner Bio-One - Knowledgebase

PapilloCheck

To obtain paraffin embedded sections is painful and difficult. Whether the PapilloCheck kit can be used for sample from any other source? Is there any reference of any research group using the PapilloCheck chip with DNA sample from any other source?

Currently the possibility to use paraffin embedded samples is being tested. ...

Why spotting every chip-probe 5 times?

Jede HPV Probe wird in 5 Repliken (also 5 Spots) aufgetragen. Dies minimiert das Risiko, dass Verunreinigungen des Arrays wie z.B. Staubpartikel etc. oder Luftblasen während der Hybridisierung, Grund für ein negatives Ergebnis wären. Die...

Will the panel of detectable HPV viruses be extended?

NO! The present virus panel is covering more than 98% of all cervical cancers, screened for the presence of HPVs so far. A comparison of the HPV panels with other kits (E.g. Genomica, Roche) shows, that the extended HPV panels of those...

Is it possible to use PapilloCheck also for HPV-Typing of Parrafin embedded sections?

YES! This is possible. The protocol for that will be included into the "Extraction protocols for HPV-detection" soon.

Is semiquantification of HPVs possible?

YES and NO! With PapilloCheck, (semi)quantification of the viral load within a sample is possible but is restricted to multiple HPV infections. In this case, the values for the SNRs will reflect the ratio between the different HPVs within...