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Separation
Answers to your questions on the topic Separation can be found here.

Separation

When using Leucosep®, where do the polymorphonuclear leucocytes end up?

After separation of whole blood with Leucosep® several layers (bands) are forming. ...

Can a blood sample of only 10 ml be separated with OncoQuick?

Blood samples of less than 15 ml may be diluted with buffered physiological saline to yield 15 ml total volume. However, separation will only be successful if the total volume of blood cells reaches 5 ml.

Does Oncoquick require a specific centrifuge?

OncoQuick works with all standard centrifuges that fulfill the following specifications: ...

Can the Oncoquick be re-used several times?

No, OncoQuick is for single use only.

Is it possible to use Leucosep® to perform the enrichment of tumor cells from peripheral blood?

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What is the advantage of screening patient blood for tumor cells and not bone marrow?

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Which are the advantages of OncoQuick® as compared to so called combined approaches using immunglobulins and density gradients?

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Which are the advantages of OncoQuick® vs. conventional density gradient centrifugation (e.g. Ficoll)?

- Simplified filling due to porous barrier ...

Which are the advantages of OncoQuick® compared to immunomagnetic beads (MACS, Dynabeads)?

- Significantly faster enrichment of tumor cells (45 min vs. 90-120 min) ...

Proliferating cells differ in size. Does this phenomenon have any effect on the enrichment of tumour cells with OncoQuick®?

No. ...

Can OncoQuick® be used for the enrichment of circulating tumour cells in mice blood?

No, not at present.

What is the explanation for the term Minimal Residual Disease (MRD)?

MRD describes a certain stage of a tumoral disease characterized by disseminated tumor cells remaining in the patient's body after surgical resection or chemo-/radiotherapy of a primary tumor. Very often these remaining cells are resistant...

Is it possible to enrich tumor cells from minced tumor tissue by means of OncoQuick®?

No! ...

Can I use OncoQuick® to perform the enrichment of leukemic cells from whole blood or bone marrow?

This does not work since the buoyant density of leukemic and hematopoietic cells is similar.

Which are the advantages of OncoQuick®?

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Is OncoQuick® a diagnostic tool?

No! ...

Can OncoQuick® be used for the enrichment of circulation tumour cells in patients with Melanoma?

Malignant Melanomas are highly invasive tumours which start to disseminate cells into blood and lymph at early stages. OncoQuick® has successfully been used to enrich tyrosinase-positive Melanoma cells from whole blood. ...

Is it sufficient to enumerate 10,000 cells per ml blood to securely diagnose MRD (minimal residual disease)?

In this case only about 0.025 µL blood would be examined – a volume far to little for secure diagnosis. OncoQuick® provides the opportunity to examine 25 ml whole blood for residual tumour cells on only one slide.