Greiner Bio-One - Knowledgebase

Immunology/HLA

Answers to your questions on the topic Immunology/HLA can be found here.

Immunology/HLA

I would like to clarify about the usage of flat bottom 96 well plates from your company.
Is it suitable it for coating in ELISA ?

Yes, our 96 well microplates or strip plates which are sold under the brand name MICROLON®, FLUOTRAC™ or LUMITRAC™ can be used for coating proteins in ELISA applications. ...

I'm currently using your Lumitrac 600 96-well plates for our ELISA assay. We'd like to move to a 384-well format fluorescent detection. Is the Fluortrac 600 384-well plate the best option for us?

Lumitrac are white microplates. Fluotrac stands for black microplates. As the assay was developed in white high binding plates (Lumitrac 600, 96 well) our recommendation would be to switch to white 384 well Lumitrac microplates. The...

Question concerning ELISA plates: What is the difference between Half-Area and F-Bottom?

Greiner Bio-One offers microplates with two different F-Bottom formats - Standard F-Bottom and F-Bottom Chimney Well. Half Area is equal to the F-Bottom format, the smaller well size reduces the sample volume about 50 %. ...

When "Microlon" (like "Microlon 600") appears in the description for a plate, does it mean that the plate is coated with a substance called "Microlon 600"? If not, what does it mean?

In general, immunonolgical microplates from Greiner Bio-One are not coated. ...

What are the differences between "high binding" plates and "medium binding" plates?

High binding polystyrene surfaces are hydrophilised using a special method of physical treatment. They display a higher binding capacity for polar molecules. ...

Can TC treated plates be used for EIA?

It is not recommended to use cell culture treated plates (TC treated microplates / Cellstar microplates) in ELISAs. The cell culture treatment (TC treatment) of polystyrene creates highly charged surfaces with high affinity to all kind of...