Greiner Bio-One - Knowledgebase

HTS-/Microplates

Answers to your questions on the topic HTS- and Microplates can be found here.

HTS-/Microplates

What is the difference between µClear and crystal clear?

µClear® is the brand name of our black and white clear film bottom microplates. ...

What kind of microplate and with which treatment would you recommend to use for a cell based assay where the cells do not need to attach to the microplate surface (assay with non adherent cells)?

If sterility is not a requirement for the assay the non treated med binding plates can be used. ...

What is the bottom thickness of your solid bottom transparent microplates?

The bottom thickness of the most frequently used solid bottom transparent microplates is: ...

Which sterilisation method is used at Greiner to sterilise TC microplates, e.g. Cat.-No. 677 180?

Those products are sterilised by ionising radiation with a Sterility Assurance Level (SAL) of 10-3. The validation of the irradiation dose is according to EN 552, ISO 11137, ANSI/AAMI ST31/ST32.

How much is the recommended working volume for 384 Well microplates (Cat.-No. 781182)?

For 781 182 the recommended working volume is between 10 and 130 µl - depends on the requirements and applications of the customer. ...

I am interested in trying out your 384-well microplate (Cat-No.784080).
Is this a viewplate (clear bottom to visualise cells), and how much does a case cost?

The microplate with Cat.-No. 784080 is a 384 well white Small Volume™, solid bottom, cell culture treated, sterile microplate with lid. The plate does not have a clear film it is a solid bottom microplate. ...

I am looking for glass bottom plates that I can use to grow biofilms and image them under the fluorescent microscope. Does Greiner Bio-One have 48-well glass bottom plates in the product portfolio?

No we do not have 48 well glass bottom plates in our product portfolio, but the following glass bottom plates (Sensoplates™ ) are available from Greiner Bio-One GmbH: ...

Does Greiner Bio-One sell bottomless 24 or 96 well microplates, preferably in polypropylene?
If yes, do you also have information about how to attach it to a modified glass surface?

No, we do not sell bottomless polypropylene microplates. But on request we sell bottomless 24, 96, 384 and 1536 well polystyrene microplates. The glassbottom can be attached by gluing. However this might be difficult and needs the testing...

Solubilized NADH linked enzyme assays are often commonly performed using absorbance at 340 nm (near UV). The low protein binding characteristics of polypropylene plates make them ideal for biochemical work. What are the spectral properties of your clear-bottom polypropylene microplates?

We do not offer clear film bottom polypropylene microplates. The standard solid bottom polypropylene microplates might have a relatively high background in the range of 0,3 to 0,4 AU at 340 nm. But clear bottom polystyrole microplates are...

What is the quantity of protein necessary for the saturation of microplate 675097.

What is the inner radius and height of each well on 96 well microplate, UVstar, chimney well; item # 655801?

Well geometry of the 96 Well UV-Star® Microplate: ...

What is the advantage of a µClear plate over a standard polystyrene plate?

The µClear® microplates are clear bottom microplates with a thin polystyrene film bottom (190 µm +/- 20 µm for a 96 and 384 well formats and 75 µm +/- 10 µm for the 1536 well format). ...

Does Greiner Bio-One have microplates for measurement of DNA?

Yes! For the determination of nucleic acid and protein concentrations at 260 nm or 280 nm without background interference the UVStar® microplates are an ideal alternative to expensive and fragile quartz glass microplates or cuvettes. ...

What means V-, U- and F-bottom and when should I use which microplate?

96 well microplates are in available with different well designs: ...

When should I use a white or black microplate?

White microplates are usually used for luminescence measurements and black microplates for fluorescence measurements. The critical properties in these methods, such as background, autofluorescence or crosstalk are considerably improved by...

Can TC treated plates be used for EIA?

It is not recommended to use cell culture treated plates (TC treated microplates / Cellstar microplates) in ELISAs. The cell culture treatment (TC treatment) of polystyrene creates highly charged surfaces with high affinity to all kind of...

Can Greiner Bio-One microplates be autoclaved?

Microplates made out of polystyrene do not withstand the high temperatures of the autoclaving process (specified thermal stability of polystyrene = -20°C - + 60°C) ...