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Cell and Tissue Culture
Answers to your questions on the topic Cell and Tissue Culture can be found here.

Cell and Tissue Culture

What is the difference between µClear and crystal clear?

µClear® is the brand name of our black and white clear film bottom microplates. ...

Which is the appropriate pore size selection for a migration assay?

Relative to the size of the investigated cells, the size of the pores in the membrane must be small enough to avoid the passive passage of cells, but large enough to allow their active migration. Customarily, 3 µm-pores are applied for...

I aim to establish a migration assay with human glioma cells. After migration over 16 h the migratory cells on the membrane underside should be characterised immunocytochemically with an anti EphB4 antibody.
Can you provide recommendations for this experiment? Will it be possible to visualise the staining in bright field microscopy?

ThinCert™ cell culture inserts are well suited for immunocytochemical staining. In principal bright field microscopy as well as fluorescence microscopy may be applied to the insert membrane. ...

Can Greiner provide Cat.No. 675098 with a Poly D- or L-Lysin coating? If so, what ist the minimum order quantity and what is the delivery time?

Cat.No. 675 098 can be delivered with a Poly-D-Lysin/ Poly-L-Lysin coating.  ...

I want to culture neurospheres on Ultra Low Adhesion plates. Do you have any suitable products for this?

No, actually we do not manufacture special produducts with ultra low adhesion surface properties for cells (e.g. neurospheres).

What is the difference between Poly-D- and Poly-L-Lysine-coated microplates?

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I want to buy a 96-well plate, which can be used in cell culture and detect luminescent signal, so the well must have a clear bottom and a opaque wall to avoid crosstalk. And it should be sterilized. Which product should I choose? Is it #655098 or #655083?

What means "µCLEAR"?

Microplates with the part number #655 083 are cell culture treated solid bottom microplates (sterile with lid) Microplates with the part number #655 098 are cell culture treated clear bottom (µClear) microplates (sterile with lid): So the...

Are their differences in the coating of the Products No 633180 and 633171?

Cat.-No. 633180 is a petri dish for bacteriology and not suitable for cell culture. As this dish has not a treated surface, cell adhesion is not possible. Furthermore, cell culture products like Cat. No. 633171 are sterile and regularly...

Can cells be grown on tissue culture inserts for use in transmission electron microscopy? Which type of insert is recommended for this application?

ThinCert™ cell culture inserts are well suited for use in transmission electron microscopy. In principle each pore size and pore density may be used (0.4 µm, 1.0 µm, 3.0 µm, 8.0 µm, translucent and transparent membranes). For choice of the...

Is the AutoFlask up to the ANSI standard?

The external dimensions comply with ANSI Standards to render the flask suitable for use on a wide range of cell culture and liquid handling systems.

Can quadriPERM be used for adherent cell culture?

quadriPERM can be used for adherent cell culture only in combination with coated and uncoated glass and plastic slides. Furthermore flexiPERM can be used for the cultivation for suspension culture cells.

Why do ThinCert™ cell culture inserts have an eccentrically positioned in-well design?

The eccentric position of ThinCert™ cell culture inserts conveniently provides a large opening for accessibility to pipettes, thus allowing an easy exchange of culture medium.

How can I find the large opening of the ThinCert™ cell culture insert for accessibility to pipettes?

The large opening for accessibility to pipettes is located opposite of the broad flange of the cell culture insert.

Which are the advantages of the hanging geometry of ThinCert™ cell culture inserts?

The hanging geometry of ThinCert™ cell culture inserts ensures that a distance is kept between the insert underside and the well bottom, thus avoiding any damage of cells in the lower compartment during co-culture experiments. Furthermore,...

What material is the ThinCert™ membrane made of?

The capillary pore membrane of ThinCert™ cell culture inserts is made of PET (Polyethylene therephthalate).

Why has PET rather than PC been chosen as the membrane material of ThinCert™ cell culture inserts?

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What is the difference between transparent and translucent membranes of ThinCert™ cell culture inserts?

Transparent membranes comprise a lower pore density as compared to translucent membranes. With the different pore densities also the optical appearance changes. Transparent membranes are optimised for light microscopy.

How can the ThinCert™ membrane be further processed?

The membrane of ThinCert™ cell culture inserts can be easily detached from the insert housing using a scalpel. Due to its anti-curling effect and its high solvent resistance the membrane may be further processed in standard histological and...

Is the lower side of the ThinCert™ membrane tissue culture treated?

As well as the upper side the lower side of the membrane is tissue culture treated. The tissue culture treatment helps migratory or co-cultivated cells to stay attached to the lower membrane surface. ...

How thick is the ThinCert™ membrane?

The thickness of the membrane of ThinCertTM cell culture inserts is graduated according to the size of the integrated pores. It is: 22.6 µm for 0.4 µm pores, 22 µm for 1.0 µm pores, 20 µm for 3.0 µm pores and 15 µm for 8.0 µm pores.

Which ThinCert™ inserts are suitable for transport studies?

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Which ThinCert™ cell culture inserts are suitable for co-culture experiments?

Usually membranes with 0.4 µm or 1.0 µm are applied in co-culture experiments. ...

Which ThinCert™ cell culture inserts are suitable for migration and invasion studies?

In a migration assay the migratory cells migrate actively through the capillary pores of the insert membrane. The pore diameter must therefore be large enough to allow the active migration of the cells, but small enough to prevent any...

Which ThinCert™ inserts are suitable for organotypic culture and tissue reconstruction in vitro?

In many cases the best results for organotypic culture have been obtained with ThinCert™ cell culture inserts with 0.4 µm and 1.0 µm. Sometimes 3.0 µm pores have been successfully applied.

Can ThinCert™ cell culture inserts be used for TEER measurements?

Yes, the low basic resistance of the membrane of ThinCertTM cell culture inserts guarantees their excellent performance in TEER measurements.

How can flexiPERM be sterilized?

The flexiPERM can be sterilized according the following protocol: ...

Do I need any glue to fixate flexiPERM on the surface??

Glue is not required to fix flexiPERM onto the surface. Due to the adhesive properties of the material, flexiPERM is sticking to all smooth surfaces like glass and plastic.

Is flexiPERM supplied with a lid?

No. flexiPERM is used in combination with TC dishes or quadriPERM. The cover of these vessels also generates the cover for flexiPERM.

Please specify the thickness of the lumox foil in multiwell plates and dishes?

The thickness of the lumox foil in 24-, 96- and 384-well plates is 50 µm. The as 5m and 40 m-roll available lumox foil and the lumox foil used in dishes is 25µm thick.

What kind of coatings does Greiner Bio-One offer for tissue culture products?

Greiner Bio-One is offering various products for tissue- and cell culture: ...

Can my roller bottles be shipped by air?

The PET roller bottles CAN NOT be shipped by air. They are sensitive to changes in air pressure and may implode if the cabin loses pressure. The polystyrene bottles can be shipped by air.

What is the maximum speed at which Greiner Bio-One 15 ml centrifuge tubes can be spun?

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